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KMID : 0644319990050010061
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Journal of Korean Oriental Oncology
1999 Volume.5 No. 1 p.61 ~ p.75
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Anti-metastastic Effects of Xuefuzhuyutang
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李眞華/Lee, Jin-Hwa
沈範相/安圭錫/崔昇勳/Shim, Bum-Sang/Ahn, Kyoo-Seok/Choi, Seung-Hoon
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Abstract
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To examine the effect of XuehdRUIng on the metastasis of cancer, the following experiments were
carried out. Before the main experimnts, the cytotoxicity was measured by putting Xuefuzhuyutant sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. Weste blotting was carried out to examine the changes of Fos, Jun, Ets, Erk, md JNK. In vitro invasion assay
with transwells coated by collagen and mangel was carried out. From the above reams the following
conclusions were obtained.
1. The eXperimental result about cytotoxicity of Xuefuzhuyutang agaitst HTlO8O was a below. The
stained cell count after beingtreated by by Xuefuzhuyutang sample 4M㎍/㎖ for 24 hours was 0.9% of total cells, and the stained cell count by Xuefuzhuyutang sample 100㎍/㎖ was 1.5% of total
cells. Both were near the level of control group which showed O.6% stained.
2. Thee result of collagenase assay was as below. In Xuefuzhuyutang sample 400㎍/㎖, MMP2 was
reduced as compared with TPA control group, and the band of MMP-9 induced by TPA
disapappeared. In Xuefuzhuyutang samle 800㎍/㎖ both bands of MMP-2 and MMP-9 diSamared.
3. The results of westem blots for Jun, Fos, Ets, Erk, JNK were a below. In Xuefuzhuyutang sample 200㎍/㎖,
Ets was reduced, and Jun, Fos were increased.
4 The result of invasion assay was as below. The number of cells which migrated gross trayswell
membrane in Xuefuzhuyutang-treated group was less than that of control(+TPA) group.
From the above results, it was concluded that Xuefuzhuyutang might inhibit the activity of
collagenase not by the MMP-2, MMP-9 promoter but by the Other way.
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